A multipath-output-supporting evidence-based acupuncture and moxibustion acupoint therapeutic and detecting instrument and a using method thereof. The therapeutic and detecting instrument comprises a CPU core unit, a communication module, an audio module and a plurality of detecting, diagnosis and treatment circuits. The detecting, diagnosis and treatment circuit comprises an acupoint detecting unit and an acupoint acupuncture and moxibustion unit. The acupoint detecting unit comprises a resistance detecting circuit and an A/D digital converting circuit. The acupoint acupuncture and moxibustion unit comprises a D/A pulse current generating circuit and an amplifying circuit. The CPU core unit comprises an ARM processor, a memory, an auxiliary circuit and an evidence-based acupuncture and moxibustion therapeutic system, and the ARM processor is connected with the evidence-based acupuncture and moxibustion therapeutic system.