- 专利标题: Cell-free system for converting methane into fuel and chemical compounds
-
申请号: US14137524申请日: 2013-12-20
-
公开(公告)号: US09611487B2公开(公告)日: 2017-04-04
- 发明人: William Jeremy Blake , James R. Swartz
- 申请人: GreenLight Biosciences, Inc.
- 申请人地址: US MA Medford
- 专利权人: GreenLight Biosciences, Inc.
- 当前专利权人: GreenLight Biosciences, Inc.
- 当前专利权人地址: US MA Medford
- 代理机构: Wolf, Greenfield & Sacks, P.C.
- 主分类号: C12P7/16
- IPC分类号: C12P7/16 ; C12P7/40 ; C12P7/64
摘要:
The present disclosure relates, in some aspects, to cell-free methods and systems for large-scale conversion of methane to isobutanol, comprising combining, in a bioreactor at elevated pressure, methane, oxygen, and cell lysates containing methane monooxygenase, methanol dehydrogenase, and enzymes that catalyze the conversion of formaldehyde to isobutanol, to form a cell-free reaction mixture, and incubating under suitable conditions the cell-free reaction to convert methane to isobutanol.