A display substrate, a method for manufacturing the same, and a liquid crystal display device are disclosed. In the present disclosure, an alignment film layer is formed on the display substrate and a shield by placing the shield on the display substrate and using the shield as a barrier, wherein, an enclosed region is enclosed by the shield, a inner edge of the shield is located between the display region and a sealant region of the display substrate, and an outer edge of a non-display region of the display substrate is located within a region where the shield is located. Then the shield is peeled off and an aligning process is performed to form an alignment layer.