A pixel structure, a driving method thereof and a display device are provided. The pixel structure includes a plurality of closely arranged repeating groups, and each of the repeating groups includes linearly arranged square pixel units of different colors. Each of the square pixel units in each of the repeating groups is formed by two sub-pixels with a same color and a same shape; and two sub-pixels in adjacent square pixel units have different arrangement modes. The repeating groups disposed on two adjacent parallel straight lines are staggered by a distance of one and a half square pixel units. With such a pixel structure, input information is subjected to brightness redistribution and intensively outputted to the actual physical positions, the optional switching of the sub-pixels can be applied on the premise of not reducing the pixel size, and hence the resolution of the display image can be improved.