Methods and apparatuses are provided for controlling reference signal (RS) transmission between a user equipment (UE) and a base station in a wireless communication system. A method includes transmitting, to the UE, downlink control information (DCI) including RS request information. The RS request information includes one or two binary elements based on a DCI format. If an RS transmission triggered by the RS request information is to occur in a same subframe as another RS transmission previously configured to the UE from the base station, the UE transmits the RS triggered by the RS request information.