A color filter substrate is disclosed. The color filter substrate includes a glass substrate, and an RGB color filter layer. The RGB color filter layer includes a plurality of optical filter columns, and each optical filter column includes a plurality of optical filter units in two colors. In addition, adjacent optical filter columns form an optical filter group, optical filter units of one of the colors in each optical filter group are arranged in a zigzag pattern, optical filter units arranged in the zigzag pattern are of different colors in adjacent optical filter groups, and the optical filter units are rectangular.