Invention Grant
- Patent Title: Multi-primer amplification method for tagging of target nucleic acids
-
Application No.: US14180262Application Date: 2014-02-13
-
Publication No.: US09677119B2Publication Date: 2017-06-13
- Inventor: Andrew May , Peilin Chen , Jun Wang , Fiona Kaper , Megan Anderson
- Applicant: Fluidigm Corporation
- Applicant Address: US CA South San Francisco
- Assignee: Fluidigm Corporation
- Current Assignee: Fluidigm Corporation
- Current Assignee Address: US CA South San Francisco
- Agency: Weaver Austin Villeneuve & Sampson LLP
- Agent Emily M. Haliday
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C07H21/02 ; B01L3/00 ; B01L7/00

Abstract:
In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.
Public/Granted literature
- US20140272952A1 MULTI-PRIMER AMPLIFICATION METHOD FOR BARCODING OF TARGET NUCLEIC ACIDS Public/Granted day:2014-09-18
Information query