Invention Grant
- Patent Title: Animal product-free culture medium and a process for producing a supernatant of clostridium comprising one or more collagenolytic and gelatinolytic proteases
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Application No.: US14404155Application Date: 2013-05-29
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Publication No.: US09725692B2Publication Date: 2017-08-08
- Inventor: Marcos Castanheira Alegria , Lucidio Cristovao Fardelone , Marina Baiochi Riboldi Delalana , Josef Ernst Thiemann , Spartaco Astolfi Filho , Roberto Carlos Debom Moreira , Ogari de Castro Pacheco
- Applicant: CRISTALIA PRODUTOS QUIMICOS FARMAEUTICOS LTDA.
- Applicant Address: BR São Paulo
- Assignee: CRISTALIA PRODUTOS QUIMICOS FARMACEUTICOS LTDA.
- Current Assignee: CRISTALIA PRODUTOS QUIMICOS FARMACEUTICOS LTDA.
- Current Assignee Address: BR São Paulo
- Agency: Roberts Mlotkowski Safran Cole & Calderon, P.C.
- Priority: BR102012013110 20120531
- International Application: PCT/BR2013/000192 WO 20130529
- International Announcement: WO2013/177647 WO 20131205
- Main IPC: C12N1/20
- IPC: C12N1/20 ; C12N9/52 ; C12P1/04 ; A61K38/48
Abstract:
The present invention refers to a culture medium and a process for producing Clostridium collagenolytic and gelatinolytic proteases. The present invention includes an animal product-free culture medium for C. histolyticum comprising non-animal derived peptones, preferably vegetable derived peptones, yeast extract and the amino acids cysteine and arginine. The present invention also includes a process for producing a supernatant of Clostridium histolyticum liquid culture comprising one or more collagenolytic and gelatinolytic proteases, and pharmaceutical composition comprising as active ingredient a supernatant of the Clostridium cultures described herein.
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