A chromatographic medium having a separating agent layer, which is used to separate target substances, a filling agent layer, which is used to fix the target substances before the target substances are separated, and a permeation layer, which is used to enable permeation of the target substances separated by the separating agent layer, wherein the filling agent layer comes into contact with the separating agent layer via a plane that intersects the direction of development of the target substances in the chromatographic medium and is positioned on the upstream side in the direction of development, the separating agent layer exhibits separability of the target substances and optical responsiveness to ultraviolet rays, and the permeation layer exhibits an optical responsiveness that is different from those of the target substances and the separating agent layer.