A transflective liquid crystal display panel, a manufacturing method thereof and a display device are disclosed. The transflective liquid crystal display panel includes: a first substrate (1) and a second substrate (2) disposed oppositely and a liquid crystal layer (3) between the first substrate (1) and the second substrate (2). The first substrate (1) and the second substrate (2) include transmissive areas and reflective areas. Transmissive areas of the first substrate (1) are provided with a first homogenous alignment layer (11), transmissive areas of the second substrate (2) are provided with a second homogenous alignment layer (21), and the alignment direction of the first homogenous alignment layer (11) and the alignment direction of the second homogenous alignment layer (21) have a predetermined angle. Reflective areas of the first substrate (1) are provided with a third homogenous alignment layer (12) and reflective areas of the second substrate (2) are provided with a homeotropic alignment layer (22). This can realize a transflective liquid crystal display panel with simple structure.