An alignment film and a fabrication method thereof, a liquid crystal panel and a display device, relate to a field of liquid crystal display technology, which can avoid causing static electricity and generating dust during a rubbing process, as well as unevenness of alignment. The method comprises: dispersing discotic liquid crystal molecules containing hydrophobic branched chains in a solvent, to prepare a discotic liquid crystal molecule solution (101); and applying the discotic liquid crystal molecule solution on a substrate, and obtaining the alignment film after removing the solvent (102).