The present application discloses a circuit board positioning device, comprising: a beam, a first side of the beam forming a circuit board supporting surface, and the first side being provided with a first fixture block and a second fixture block, a first positioning slot being formed on the first fixture block, a second positioning slot being formed on the second fixture block, the first positioning slot and the second positioning slot being used for limiting positions of two opposite side edges in the circuit board; a snap-fit, one end of the snap-fit being rotatablely mounted on the beam around one end of the beam; a lock mechanism being provided between the snap-fit and the beam for locking the snap-fit and the beam when the snap-fit is in a closed position, and a side of the snap-fit facing towards the beam being provided with at least one elastic pressing sheet protruding towards the beam when being in the closed position. The circuit board positioning device can protect the circuit board from being damaged when shock occurs.