A sheet conveyance apparatus is equipped with a stacking portion on which sheets are stacked, a rotator conveying a sheet by rotating in a contact state with the sheet stacked on the stacking portion, a separating member pressing the rotator and separating the sheets one by one at a contact portion where the rotator and the separating member contact each other, and a holder holding the separating member. A guide portion is rotatably provided with the holder and capable of guiding a front end of the sheet to the contact portion, and a regulating portion regulates the guide portion from rotating in a direction approximating the rotator in a state where the guide portion contacts against the regulating portion. The guide portion can be rotated in a direction separating from the rotator in a state where the separating member presses the rotator.