An apparatus and method are provided for transmitting a DM RS associated with a PUSCH in a communication system. The method includes allocating a CS for a DM RS of a UE, and an OCC; transmitting DCI including a plurality of IEs over PDCCHs, where each of the plurality of IEs respectively includes information indicating the CS for the DM RS and the OCC; and receiving, from the UE, the DM RS associated with the PUSCH. A DM-RS sequence is acquired based on the CS for the DM RS and the OCC identified from the information indicating the CS for the DM RS and the OCC, the CS is a value for determining a cyclic shift of a reference sequence, and the CS corresponds to a predetermined OCC, and the information indicating the CS for the DM RS and the OCC consists of 3 bits.