The present invention discloses a circuit, a display substrate and a display device, the circuit comprises a plurality of circuit modules, distances of at least one circuit module from two circuit modules adjacent thereto are a first distance and a second distance, respectively, the first distance is smaller than the second distance, and the absolute value of the difference between the second distance and the first distance is not equal to an integer multiple of the first distance. In the present invention, distances of at least one circuit module from two circuit modules adjacent thereto satisfy the above specific condition, so that at least one circuit module can be prevented from being positioned at the middle position of the standing wave field between the two circuit modules adjacent thereto, therefore, interference of standing wave to transmission signals is reduced, and signal distortion and signal attenuation are alleviated.