Invention Grant
- Patent Title: Nucleic acid analysis device
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Application No.: US14421239Application Date: 2013-07-08
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Publication No.: US09770713B2Publication Date: 2017-09-26
- Inventor: Takanobu Haga , Yoshitaka Kodama , Tomohiro Shoji , Takamichi Muramatsu , Shuhei Yamamoto
- Applicant: Hitachi High-Technologies Corporation
- Applicant Address: JP Tokyo
- Assignee: Hitachi High-Technologies Corporation
- Current Assignee: Hitachi High-Technologies Corporation
- Current Assignee Address: JP Tokyo
- Agency: Mattingly & Malur, PC
- Priority: JP2012-189423 20120830
- International Application: PCT/JP2013/068588 WO 20130708
- International Announcement: WO2014/034275 WO 20140306
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; B01L3/00 ; G01N21/64 ; B01L99/00

Abstract:
A nucleic acid analysis device which can determine a DNA sequence has a flowcell in which two or more DNA fragment clusters of two or more DNA fragments having identical nucleotide sequences are immobilized. At least a part of the flowcell is made of a transparent material. An irradiation unit irradiates a part in which the DNA fragment clusters are immobilized. The device has a lens for collecting fluorescence, and a light-detection element. A solution containing only dATP having a fluorescently modified phosphate terminal among four bases, a solution containing only dCTP having a fluorescently modified phosphate terminal among the four bases, a solution containing only dGTP having a fluorescently modified phosphate terminal among the four bases, a solution containing only dTTP having a fluorescently modified phosphate terminal among the four bases, and a buffer solution are sent sequentially to where the DNA fragment clusters are immobilized.
Public/Granted literature
- US20150202618A1 NUCLEIC ACID ANALYSIS DEVICE Public/Granted day:2015-07-23
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