A pixel driving method of a display panel is disclosed. The display panel includes a plurality of scan lines, data lines and pixels. Each of the pixel includes a first transistor with a first end coupled to the data line, and a gate end coupled to the scan line, a second transistor with a first end selectively coupled to a voltage source or current source, and a gate end coupled to a second end of the first transistor, and a light-emitting unit with a first end coupled to a second end of the second transistor. The method includes turning on the first transistors of the pixels; coupling the data lines and first ends of the second transistors to the current source; reading voltage levels of gate ends of the second transistors; and providing corresponding data voltages to the pixels according to voltage levels of gate ends of the second transistors.