A surgical simulation assembly is provided for simulating realistic surgery on animal organs or human cadavers. The surgical simulation assembly comprises a generally flat tray having a central indentation for receiving an organ, at least one drainage aperture and at least one indented trough extending from the central indentation for receiving at least one tube. The tray is assembled with a simulator heart and lungs prepped for connection to a variable speed double action air pump assembly that creates both positive pressure and a vacuum on the heart to simulate a beating heart. The tray is supported on a basket having a lower containment portion for containing fluid waste collected during the simulated surgery. The basket is elevated by risers over a base designed to support the assembly.