A method and a system for detecting sentiment of a human based on an analysis of human speech are disclosed. In an embodiment, one or more time instances of glottal closure are determined from a speech signal of the human. A voice source signal based on the determined one or more time instances of glottal closure is generated. A set of relative harmonic strengths is determined based on one or more harmonic contours of the voice source signal. The RHS is indicative of a deviation of the one or more harmonics of the voice source signal from a fundamental frequency of the voice source signal. A set of feature vectors is determined based on the RHS. The set of feature vectors are utilizable to detect the sentiment of the human.