- 专利标题: Recombinant microorganism for preparing terpenoid and method for constructing recombinant microorganism
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申请号: US14765317申请日: 2014-01-28
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公开(公告)号: US09816124B2公开(公告)日: 2017-11-14
- 发明人: Xueli Zhang , Qingyan Li , Jing Zhao , Tao Sun , Guanping Dai , Hongtao Xu , Jinlei Tang , Yanhe Ma
- 申请人: TIANJIN INSTITUTE OF INDUSTRIAL BIOTECHNOLOGY, CHINESE ACADEMY OF SCIENCES
- 申请人地址: CN Tianjin
- 专利权人: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
- 当前专利权人: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
- 当前专利权人地址: CN Tianjin
- 代理机构: Knobbe Martens Olson & Bear, LLP
- 优先权: CN201310039787 20130201
- 国际申请: PCT/CN2014/000130 WO 20140128
- 国际公布: WO2014/117639 WO 20140807
- 主分类号: C12P23/00
- IPC分类号: C12P23/00 ; C12N9/02 ; C12P5/00
摘要:
Provided are a recombinant strain for preparing a terpenoid, and method for constructing the recombinant strain. Also provided is a recombinant bacterium 1, the recombinant bacterium 1 being a recombinant bacterium obtained in order to improve the enzymatic activity of α-ketoglutarate dehydrogenase in escherichia coli or the mutant thereof. The method for improving the enzymatic activity of α-ketoglutarate dehydrogenase in escherichia coli or the mutant thereof is replacing the original regulating element of the ketoglutarate dehydrogenase gene (sucAB) in escherichia coli or the mutant thereof with any of the following regulating elements: artificial regulating element M 1-46, M1-37, and M1-93. Also provided are a plurality of recombinant bacteria. By improving the enzymatic activity of α-ketoglutarate dehydrogenase, succinic acid dehydrogenase and transaldolase therein and improving the ability of a cell to synthesize NADPH and ATP, the efficiency of the MEP pathway and the production capacity of terpenoid are improved.
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