A separation chamber type anti-surge valve may include a valve body formed with a charge air passage inlet and a charge air passage outlet, and a valve cover coupled to the valve body by a fastening member to define an empty inner space between the valve body and the valve cover, in which the inner space is divided into a diaphragm chamber and a bypass chamber by a valve guide, one side tip of a valve rod being fixed in the diaphragm chamber, a valve disc fixed to another side tip of the valve rod, and the diaphragm chamber is divided into a control pressure chamber and a normal static pressure chamber.