An image sensor including a color filter isolation layer and a method of manufacturing the image sensor. The image sensor includes a plurality of color filters that transmit light of a predetermined wavelength band to a light sensing layer. The image sensor also includes an isolation layer disposed between adjacent ones of the plurality of color filters. The isolation layer is formed of a material having a lower refractive index than a refractive index of the color filters, thus totally internally reflecting light incident on the isolation layer from one of the plurality of color filters.