An array substrate and a manufacturing method thereof, a display apparatus are disclosed. The manufacturing method of the array substrate includes a forming a conduction layer (20) for electrically connecting two adjacent pixel electrodes (104) upon forming of the pixel electrodes (104). The method further includes forming a photoresist layer (30) on a surface of a substrate with the conduction layer (20) formed thereon; through one exposure and development process, forming a first photoresist removed region (A) and a photoresist retained region (B). The first photoresist removed region (A) corresponds to a location of the conduction layer (20); the conduction layer (20) in the first photoresist removed region (A) is etched, to form a separating region (106) configured for avoiding electrical connection of pixel electrodes (104) of two adjacent pixel units; and within the separating region (106), a metal line that is non-electrically connected to adjacent two pixel electrodes (104) is formed. With the array substrate and manufacturing method thereof, damage to excessive thin film layers in the array substrate in the course of reducing bright dot defect can be avoided.