Invention Grant
- Patent Title: Ligase-assisted nucleic acid circularization and amplification
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Application No.: US14933275Application Date: 2015-11-05
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Publication No.: US09938568B2Publication Date: 2018-04-10
- Inventor: Ryan Charles Heller , Erik Leeming Kvam , John Richard Nelson
- Applicant: GENERAL ELECTRIC COMPANY
- Applicant Address: US NY Schenectady
- Assignee: GENERAL ELECTRIC COMPANY
- Current Assignee: GENERAL ELECTRIC COMPANY
- Current Assignee Address: US NY Schenectady
- Agent Rong Zhang
- Main IPC: C12P19/34
- IPC: C12P19/34 ; C12Q1/68
Abstract:
Provided herein are methods for generation and amplification of a single-stranded DNA circle in a single reaction vessel from a linear DNA without any intervening purification steps. The single-stranded DNA circle is generated via a template-independent single-stranded DNA ligation. Whole-genome amplification of linear chromosomal DNA in a single tube using ligation-assisted DNA amplification is also provided.
Public/Granted literature
- US20160053307A1 LIGASE-ASSISTED NUCLEIC ACID CIRCULARIZATION AND AMPLIFICATION Public/Granted day:2016-02-25
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