Provided are a method, apparatus and system for measuring network packet loss, the method includes: acquiring data packet measurement information measured by at least one target logical port to a traffic stream, transmitting the data packet measurement information to a measurement control point, so that the measurement control point determine the condition of network packet loss according to the data packet measurement information. As a data collecting point acquires the data packet measurement information measured by at least one target logical port to the traffic stream, and uniformly transmits the data packet measurement information to the measurement control point, when multiple target logical ports respectively perform measurement to acquire respective data packet measurement information, the measurement control point uniformly determines condition of packet loss of the traffic stream. Therefore, accurate packet loss measurement can be performed on the traffic stream under the scenario of point-to-point, point-to-multipoint or multipoint-to-multipoint networks.