An open top refrigerated display case having an ambient air automatic defrost system and a method of operating such a case. A single air conduit extends in a U-shaped formation along opposing side walls and the bottom wall of the display case. The air conduit has openings at both ends in the location of the upper portions of the side walls. Arranged within the air conduit is at least one reversible fan and a set of refrigeration coils. During a refrigeration cycle the fan circulates air through the air conduit in a first direction towards the refrigeration coils. When frost buildup within the display case has reached a certain level, the system is switched to a defrost cycle. During the defrost cycle, the fan circulates the air in the opposite direction through the air conduit and draws in ambient air from outside of the display case. Since such ambient air is of a higher temperature than the normally refrigerated air, it serves to defrost the system. The ambient air, after passing over the evaporator coils and through the air conduit, is expelled from the air conduit in a direction towards the outer side of the refrigerated case and away from the interior of the case.