A fluorescent screen of a cathode ray tube is prepared by forming a film of photosensitive sticky substance, for example, an aqueous solution of a copolymer of a complex salt of diazonium salt and zinc chloride, oN an inner surface of a face plate of the tube, exposing the film to light to render portions thereof sticky, applying a powdery substance, for example, .[.phosphors of three colors.]. .Iadd.graphite.Iaddend., onto the film to cause the powdery substance to adhere to the portions of the film rendered sticky, removing excess powder to form a film pattern of the powdery substance, causing the substance responsible for stickiness to ooze out onto the film pattern, applying another powdery substance, for example, .[.graphite.]. .Iadd.titanium oxide .Iaddend.onto the film pattern and removing an excess of another powder substance, thus forming the fluorescent screen. The invention is applicable to the manufacture of a fluorescent screen for use in a color picture tube, a monochromatic picture tube and a penetration tube.