A window actuation assembly (10) for a vehicle (5) which is structured to automatically lower at least one, but preferably all of the windows (6) of a vehicle (5) in the event that a vehicle (5) becomes partially or totally submerged in a body of water and regardless if the orientation of the vehicle (5) is up-right, inverted, on its side, etc. One or more fluid sensors are located strategically throughout the various portions of the vehicle (5) and are specifically structured activate a drive assembly for the lowering of the windows (6) upon sensing a predetermined amount of water within an associated proximity of the vehicle, to the extent that the one or more sensors are themselves at least partially submerged. The sensors are further structured not to be activated in the unlikely event that liquid inadvertently is spilled upon or otherwise applied thereto. The one or more sensors may be associated with a single drive assembly and/or independent drive assemblies associated with each of the windows (6) such that only one, or more preferably all of the windows (6) may be lowered in an emergency, submerged condition of the vehicle (5). The windows (6) may be restricted from being raised into the normally closed position once they have been lowered under emergency circumstances, in order to prevent inadvertent closing of the windows (6) by an occupant of the vehicle (5) suffering from injury or being otherwise disoriented because of the emergency.