Invention Application
WO03001181A9 DETECTION OF CLONAL T-CELL RECEPTOR-GAMMA GENE REARRANGEMENT BY PCR/TEMPORAL TEMPERATURE GRADIENT GEL ELECTROPHORESIS (TIGE)
审中-公开
通过PCR /时间温度梯度凝胶电泳（TIGE）检测克隆T细胞受体 - GAMMA基因重排
- Patent Title: DETECTION OF CLONAL T-CELL RECEPTOR-GAMMA GENE REARRANGEMENT BY PCR/TEMPORAL TEMPERATURE GRADIENT GEL ELECTROPHORESIS (TIGE)
- Patent Title (中): 通过PCR /时间温度梯度凝胶电泳（TIGE）检测克隆T细胞受体 - GAMMA基因重排
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Application No.: PCT/US0219696Application Date: 2002-06-21
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Publication No.: WO03001181A9Publication Date: 2003-12-31
- Inventor: SAMOSZUK MICHAEL , ZHU DAN
- Applicant: QUEST DIAGNOSTICS INC , SAMOSZUK MICHAEL , ZHU DAN
- Assignee: QUEST DIAGNOSTICS INC,SAMOSZUK MICHAEL,ZHU DAN
- Current Assignee: QUEST DIAGNOSTICS INC,SAMOSZUK MICHAEL,ZHU DAN
- Priority: US88622701 2001-06-21
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C07H21/02 ; C12P19/34
Abstract:
The present invention provides methods and compositions for detecting and analyzing clonal T-cell receptor (TCR) gene rearrangement using temporal temperature gradient gel electrophoresis (TTGE), which employs a gradual and uniform increase in the temperature of a constant denaturing gel to resolve different DNA molecules based on base pair composition. The present invention also provides methods and compositions for providing appropriate DNA migration markers for TTGE analysis.
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