The present invention relates to an expression vector comprising a promoter that consists of FIS binding sites, an UP element and a core promoter. Also, the inventive expression vector may additionally comprise and FIS gene, which is regulated by an inducible promoter. The inventive expression vector can maximize the expression level of a target protein of foreign origin, and can use a wide range of E. coli strains as host cells regardless of the genetic characteristics of the strains. Thus, inventive expression vector is useful in industrial fields requiring the production of various proteins.