The invention refers to a computer-based system for the acquisition, analysis, and authentication of the handwritten signature. The person that performs a handwritten signature performs a series of three-dimensional movements with a plane graphical finality. The movements generate kinetic information perceived by the special pen that the signature is performed with (the writing and digital acquisition device for the primary bio-kinetic information). The information, concordant to the afferent bio-kinetic pattern, is collected by the included MEMS type acceleration sensors in the pen. The system continues to analyze the generated information (the signals) and it determines the dynamic-biometrical characteristics, based upon the biometrical dimension of the information. The characteristics are transformed in data vectors and invariants that are stored in a database. Based on algorithm type methods, the system performs the required comparisons between the spatial kinetics of the specimens and the kinetics of the entrances and obtains distance-type answers. In statistical terms, the results are related to the entire subject database, by interpreting and sampling methods.