A dialysis system includes at least one dialysis fluid pump actuator, a disposable cassette including at least one pump chamber operable with the at least one pump actuator, the disposable cassette further including a plurality of fluid ports configured to be connected fluidly to a plurality of fluid containers, and a processor programmed to cause the at least one pump actuator to operate the at least one pumping chamber to selectively move effluent dialysis fluid to a drain container or to a sample container.