The present invention is related to novel process for production of Tricholine Citrate by reacting citric acid with choline base at high temperature to obtained more than 65 percent pure of Tricholine Citrate. Such a obtained Tricholine Citrate is used in various composition for used as an effective iron chelator and used to stabilize animal growth hormone preparations also The invention provides a novel process for of production of Tricholine Citrate by reacting Citric acid with choline base solution at high temperature and distillation of Trichoiine Citrate at the same time in single step. In the process, citric acid is reacted with choline base solution at above 50 degree Celsius by adjusting pH of solution above 8.5 de-colorizing the solution obtained by de colorizing agent. The obtained product is filtered at temperature below the 40 degree Celsius in filtration unit and then distilled in Distillation Column to obtained high purity of Tricholine Citrate.