The present invention relates to a primer specific to the genes of the new influenza A virus subtype H1N1 (the 2009 H1N1 influenza virus), to a probe and to a method for diagnosing the new influenza A virus subtype H1N1 using the primer and the probe. More particularly, the present invention relates to a primer for simultaneously diagnosing the genes of the new influenza A virus subtype H1N1 through a polymerase chain reaction, to a diagnosis kit comprising an internal control and the primer, and to a diagnosis method using the kit. The primer and the probe of the present invention can be produced into a dry kit which enables the new influenza A virus subtype H1N1 in a human body to be detected in a quick and accurate manner without causing a cross-reaction with a new influenza A virus subtype H1N1 existing in the human body, and which achieves an improvement in storage safety and which does not require any expertise for a tester when being used.