A testing device for testing adhesion seal strength of a tape to a substrate. The testing device includes a body defining a chamber having a first port in fluid communication with the chamber, a platform sealingly attached to the chamber and having an elongate opening or a plurality of openings therethrough as a second port of the chamber. The testing device further includes a frame removably mounted to the platform. The frame defines a perimeter that surrounds the second port and, when mounted to the platform, secures a test substrate thereunder against the platform with a fluid-tight seal. Methods for preparing a test sample and methods of operating the testing device are also disclosed.