发明申请
WO2017096039A1 SCALABLE METHODS FOR PRODUCING RECOMBINANT ADENO-ASSOCIATED VIRAL (AAV) VECTOR IN SERUM-FREE SUSPENSION CELL CULTURE SYSTEM SUITABLE FOR CLINICAL USE
审中-公开
生产适用于临床使用的无血清悬浮细胞培养系统中重组腺病毒相关病毒（AAV）载体的可扩增方法
- 专利标题： SCALABLE METHODS FOR PRODUCING RECOMBINANT ADENO-ASSOCIATED VIRAL (AAV) VECTOR IN SERUM-FREE SUSPENSION CELL CULTURE SYSTEM SUITABLE FOR CLINICAL USE
- 专利标题（中）： 生产适用于临床使用的无血清悬浮细胞培养系统中重组腺病毒相关病毒（AAV）载体的可扩增方法
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申请号： PCT/US2016/064414申请日： 2016-12-01
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公开(公告)号： WO2017096039A1公开(公告)日： 2017-06-08
- 发明人: QU, Guang , LU, Lin , WRIGHT, John Fraser
- 申请人： SPARK THERAPEUTICS, INC.
- 申请人地址： 3737 Market Street, Suite 1300 Philadelphia, Pennsylvania 19104 US
- 专利权人： SPARK THERAPEUTICS, INC.
- 当前专利权人： SPARK THERAPEUTICS, INC.
- 当前专利权人地址： 3737 Market Street, Suite 1300 Philadelphia, Pennsylvania 19104 US
- 代理机构： BEDGOOD, Robert
- 优先权： US62/261,815 20151201
- 主分类号： C12N5/10
- IPC分类号： C12N5/10 ; C12N7/00 ; C12N7/02 ; C12N7/01 ; A61K48/00 ; A61K31/7088 ; C12Q1/70
摘要：
Methods and compositions for transfecting cells with plasmids are disclosed. In certain embodiments, methods and compositions are disclosed in which transfection efficiency is significantly increased by contacting the cells being transduced with polyethyleneimine (PEI) that is free of nucleic acid during the transfection process. Therapeutically useful adeno-associated viral vectors generated according to the disclosed methods and compositions are also disclosed.
