An anti-inflammatory and antimicrobial ointment comprises a component A and a secondary component B. The component A comprises the following ingredients in parts by weight: 15-30 parts of a sicklepod extract; 10-20 parts of a ginkgo seed extract; 5-9 parts of a Sichuan pepper extract; 2-6 parts of a champac extract; 2-7 parts of konjac glucomannan; and 1-3 parts of chitosan oligosaccharide. The secondary component B comprises: 1-3 parts of zein; 1-3 parts of glycerol; and 0.3-1 parts of beeswax. The anti-inflammatory and antimicrobial ointment achieves moderate yet significant and long-lasting anti-inflammatory and antimicrobial effects. When applied to infected skin, such as a pustule, superficial wound infection, or eczema with bacterial infection, or a surface of a purulent wound with discharge, the ointment forms a protective thin film, thereby achieving robust anti-inflammatory and antimicrobial effects, and facilitating healing of a wound surface.