A fluid-cooled LED-based lighting fixture for Controlled Environment Agriculture (CEA) to improve energy efficiency, recycle waste heat, and support the operation of environmental sensors in a controlled agricultural environment. In one example, a lighting fixture frame mechanically supports and houses respective components of the lighting fixture and includes a light spine to mechanically couple the lighting fixture to a support structure. One or more coolant pipes formed from copper and coupled to the lighting fixture frame conduct a fluid coolant through the lighting fixture to remove heat. The lighting fixture comprises one or more LED modules to emit light, one or more onboard sensors and/or cameras, wireless communication functionality, and multiple electrical power and communication ports to facilitate interconnection of the lighting fixture in a variety of controlled agricultural environments. In some examples, the lighting fixture includes a multispectral imaging system to acquire multispectral images of the environment.