This production method of an enzyme stabilizer comprises: steps (1) to (3) for obtaining lignin derivatives by acid solvolysis using a lignocellulosic biomass and a polyalkylene glycol; step (4) for adding a second acid to the liquid fraction obtained in step (3); step (5) for obtaining a liquid fraction by solid-liquid separation of the acidic liquid; step (6) for allowing a synthetic adsorbent having specific properties to adsorb a hydrophilic lignin derivative in the liquid fraction; step (7) for individually separating water and the polyalkylene glycol from the solution after the step (6) by distillation and reusing the same; step (8) for contacting an organic eluent with the synthetic adsorbent to desorb the hydrophilic lignin derivative and collecting the eluate; step (9) for purifying the hydrophilic lignin derivative from the eluate by distillation; and step (10) for reusing the separated organic eluent in step (8).