An application of a higher fatty alcohol in preparation of a formulation for improving a gene transcription level associated with synthesis pathways of jasmonic acid, cutin, and wax of corn. By using an aqueous emulsion containing the higher fatty alcohol, the synthesis pathways of cutin, wax, and jasmonic acid in a corn metabolic pathway are affected, the rust resistance, pest resistance, and drought resistance of the corn can be effectively improved, and the yield of the corn is increased.