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1.发明公开
公开(公告)号：EP1248680A1
公开(公告)日：2002-10-16
申请号：EP00905525.2
申请日：2000-01-04
IPC分类号： B03C1/28 , B03C1/01 , G01N33/543
摘要： An apparatus and method for carrying out the affinity separation of a target substance from a liquid test medium by mixing magnetic particles having surface immobilized ligand or receptor within the test medium to promote an affinity binding reaction between the ligand and the target substance. The test medium with the magnetic particles in a suitable container is removably mounted in an apparatus that creates a magnetic field gradient in the test medium. This magnetic gradient is used to induce the magnetic particles to move, thereby effecting mixing. The mixing is achieved either by movement of a magnet relative to a stationary container or movement of the container relative to a stationary magnet. In either case, the magnetic particles experience a continuous angular position change with the magnet. Concurrently with the relative angular movement between the magnet and the magnetic particles, the magnet is also moved along the length of the container causing the magnetic field gradient to sweep the entire length of the container. After the desired time, sufficient for the affinity reaction to occur, movement of the magnetic gradient is ended, whereby the magnetic particles are immobilized on the inside wall of the container nearest to the magnetic source. The remaining test medium is removed while the magnetic particles are retained on the wall of the container. The test medium or the particles may then be subjected to further processing.
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2.发明公开
公开(公告)号：EP0810905A1
公开(公告)日：1997-12-10
申请号：EP96905542.0
申请日：1996-02-16
摘要： An apparatus and method for carrying out the affinity separation of a target substance from a test medium operates by mixing magnetic particles having surface immobilized ligand or receptor with the test medium to promote affinity binding reaction between the ligand and the target substance. The test medium with the magnetic particles in a suitable container is removably mounted in an apparatus of the present invention and magnetic field gradient is created in the test medium. This gradient induces the magnetic particles to move relative to the test medium, thereby effecting mixing. Depending on the embodiment employed, the mixing is achieved either by the movement of an electromagnet or a permanent magnet relative to a stationary container or the movement of the container relative to a stationary magnetic source. After sufficient time for the affinity reaction to occur, the movement of the magnetic gradient is ended, whereby magnetic particles are immobilized on the inside wall of the container nearest to the magnetic source. The remaining test medium is removed while the magnetic particles are retained on the walls of the container and the test medium or the particles subjected to further processing.
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3.发明公开Selectively ion-permeable electrodes for analyzing selected ions in aqueous solution 失效选择性离子可渗透电极，用于在水溶液中某些离子的调查。
公开(公告)号：EP0231476A1
公开(公告)日：1987-08-12
申请号：EP86116840.9
申请日：1986-12-03
IPC分类号： G01N27/30
CPC分类号： G01N27/3335
摘要： he electrode comprises an internal reference half-cell at stable potential comprising a conductive sheet, e.g. of metal or carbon, covered by a redox system. The redox system comprises a thin dehydrated layer of Prussian blue deposited by contact between the sheet and a mixture of alkaline ferrocyanide and a ferric salt.
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4.发明公开Apparatus and method for processing magnetic particles 审中-公开Vorrichtung und Verfahren zur Behandlung magnetischer Partikeln
公开(公告)号：EP1441225A1
公开(公告)日：2004-07-28
申请号：EP04250428.2
申请日：2004-01-27
IPC分类号： G01N33/543 , B03C1/28 , B03C1/01 , G01N35/00
CPC分类号： B03C1/01 , B03C1/0332 , B03C1/0335 , B03C1/24 , B03C1/288 , B03C2201/18 , B03C2201/26 , G01N35/0098
摘要： An apparatus is described for mixing and separating magnetic particles comprising:
at least one linear array of containers (135; 145; 154A);
each container (135; 145; 154A) having a liquid and said particles therein;
at least two linear arrays (-; 146A, 146B; 151, 152) of magnetic sources (134; 147; 155) arranged in a linear pattern near said array of containers (135; 145; 154A);
means providing linear movement between said one array of containers (135; 145; 154A) and said two arrays (-; 146A, 146B; 151, 152) of magnetic sources (134; 147; 155); and
means for stopping said linear movement whereby said particles become immobilized as a relatively compact mass aggregate on the inside walls of said containers (135; 145; 154A) nearest said magnetic sources. Methods for mixing and separating particles are also described.摘要翻译： 描述了用于混合和分离磁性颗粒的装置，包括：容器（135; 145; 154A）的至少一个线性阵列; 每个容器（135; 145; 154A）具有液体并且其中的所述颗粒; 在所述容器阵列（135; 145; 154A）附近布置成线性图案的至少两个线性阵列（ - ; 146A，146B; 151,152）; 意味着在所述一组容器（135; 145; 154A）和所述磁源（134; 147; 155）的所述两个阵列（ - ; 146A，146B; 151,152）之间提供线性运动; 以及用于停止所述线性运动的装置，其中所述颗粒在最靠近所述磁源的所述容器（135; 145; 154A）的内壁上被固定为相对紧凑的质量集料。 还描述了混合和分离颗粒的方法。
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5.发明授权
公开(公告)号：EP0810905B1
公开(公告)日：1998-11-04
申请号：EP96905542.5
申请日：1996-02-16
IPC分类号： B03C1/28 , B03C1/24 , B03C1/01 , G01N33/543
摘要： An apparatus and method for carrying out the affinity separation of a target substance from a test medium operates by mixing magnetic particles having surface immobilized ligand or receptor with the test medium to promote affinity binding reaction between the ligand and the target substance. The test medium with the magnetic particles in a suitable container is removably mounted in an apparatus of the present invention and magnetic field gradient is created in the test medium. This gradient induces the magnetic particles to move relative to the test medium, thereby effecting mixing. Depending on the embodiment employed, the mixing is achieved either by the movement of an electromagnet or a permanent magnet relative to a stationary container or the movement of the container relative to a stationary magnetic source. After sufficient time for the affinity reaction to occur, the movement of the magnetic gradient is ended, whereby magnetic particles are immobilized on the inside wall of the container nearest to the magnetic source. The remaining test medium is removed while the magnetic particles are retained on the walls of the container and the test medium or the particles subjected to further processing.
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