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    A group of esterases for the enantioselective production of fine and speciality chemicals
    2.
    发明公开
    A group of esterases for the enantioselective production of fine and speciality chemicals 有权
    一组用于精细和特种化学品对映选择性生产的酯酶

    公开(公告)号：EP2385109A2

    公开(公告)日：2011-11-09

    申请号：EP11002834.7

    申请日：2007-05-03

    申请人： B.R.A.I.N. Biotechnology Research And Information Network AG

    发明人： Liebeton, Klaus ,  Eck, Jürgen ,  Bornscheuer, Uwe ,  Böttcher, Dominique ,  Langer, Peter ,  Bellur, Esen

    IPC分类号： C12N9/18 ,  C12N15/55 ,  C07K16/40 ,  C12N5/10

    CPC分类号： C12N9/18

    摘要： The present invention relates to a polynucleotide consisting of more than 30 nucleotides encoding an enzyme having carboxylesterase [E.C. 3.1.1.1] activity, wherein the coding sequence is selected from the group consisting of (a) a polynucleotide encoding an amino acid sequence as depicted in any one of SEQ ID NOs: 10, 4; 8, and 12; (b) a polynucleotide consisting of or comprising a nucleotide sequence, wherein the nucleic acid sequence is as shown in any one of SEQ ID NOs: 9, 3, 7 and 11; (c) a polynucleotide consisting of or comprising a nucleotide sequence encoding a fragment or derivative of an enzyme encoded by a polynucleotide of (a) or (b), wherein in said derivative one amino acid residue is conservatively substituted compared to the amino acid sequence of (a); (d) a polynucleotide encoding an enzyme having carboxylesterase activity which polynucleotide is at least 66% identical to a polynucleotide encoding an enzyme as shown in any one of SEQ ID NOs: 10, 4, 8 and 12; (e) a polynucleotide consisting of or comprising a nucleotide sequence the complementary strand of which hybridizes under stringent conditions to a polynucleotide as defined in any one of (a) to (b); and (f) a polynucleotide consisting of or comprising a nucleotide sequence being degenerate to the nucleotide sequence of the polynucleotide of (e); or the complementary strand of such a polynucleotide of (a) to (f).

    摘要翻译： 本发明涉及由多于30个核苷酸组成的编码具有羧酸酯酶的酶的多核苷酸[E.C. 其中所述编码序列选自（a）编码如SEQ ID NO：10,4中任一个所示的氨基酸序列的多核苷酸; 8和12; （b）由或包含核苷酸序列组成的多核苷酸，其中所述核酸序列如SEQ ID NO：9,3,7和11中任一个所示; （c）由或包含编码由（a）或（b）的多核苷酸编码的酶的片段或衍生物的核苷酸序列的多核苷酸，其中在所述衍生物中，与氨基酸序列相比，一个氨基酸残基被保守取代 （a）; （d）编码具有羧酸酯酶活性的酶的多核苷酸，该多核苷酸与编码如SEQ ID NO：10,4,8和12中任一项所示的酶的多核苷酸至少66％相同; （e）由或包含核苷酸序列组成的多核苷酸，所述核苷酸序列的互补链在严格条件下与（a）至（b）中任一项所定义的多核苷酸杂交; 和（f）由或包含与（e）的多核苷酸的核苷酸序列简并的核苷酸序列组成的多核苷酸; 或（a）至（f）的这种多核苷酸的互补链。

    A group of esterases for the enantioselective production of fine and speciality chemicals
    3.
    发明公开
    A group of esterases for the enantioselective production of fine and speciality chemicals 有权
    on on on ien ien ien ien ien ien ien ien ien ien ien ien ien ien ien ien ien ien ien ien ien

    公开(公告)号：EP2377930A1

    公开(公告)日：2011-10-19

    申请号：EP11002835.4

    申请日：2007-05-03

    申请人： B.R.A.I.N. Biotechnology Research And Information Network AG

    发明人： Liebeton, Klaus ,  Eck, Jürgen ,  Bornscheuer, Uwe ,  Böttcher, Dominique ,  Langer, Peter ,  Bellur, Esen

    IPC分类号： C12N9/18 ,  C12N15/55 ,  C07K16/40 ,  C12N5/10

    CPC分类号： C12N9/18

    摘要： The present invention relates to a polynucleotide consisting of more than 30 nucleotides encoding an enzyme having carboxylesterase [E.C. 3.1.1.1] activity, wherein the coding sequence is selected from the group consisting of (a) a polynucleotide encoding an amino acid sequence as depicted in SEQ ID NO: 6; (b) a polynucleotide consisting of or comprising a nucleotide sequence, wherein the nucleic acid sequence is as shown in SEQ ID NO: 5; (c) a polynucleotide consisting of or comprising a nucleotide sequence encoding a fragment or derivative of an enzyme encoded by a polynucleotide of (a) or (b), wherein in said derivative one amino acid residue is conservatively substituted compared to the amino acid sequence of (a); (d) a polynucleotide encoding an enzyme having carboxylesterase activity which polynucleotide is at least 66% identical to a polynucleotide encoding an enzyme as shown in SEQ ID NO: 5; (e) a polynucleotide consisting of or comprising a nucleotide sequence the complementary strand of which hybridizes under stringent conditions to a polynucleotide as defined in any one of (a) to (b); and (f) a polynucleotide consisting of or comprising a nucleotide sequence being degenerate to the nucleotide sequence of the polynucleotide of (e); or the complementary strand of such a polynucleotide of (a) to (f).

    摘要翻译： 本发明涉及由超过30个核苷酸组成的多核苷酸，其编码含有羧酸酯酶[E.C. 3.1.1.1]活性，其中编码序列选自（a）编码SEQ ID NO：6所示氨基酸序列的多核苷酸; （b）由核苷酸序列组成或包含核苷酸序列的多核苷酸，其中所述核酸序列如SEQ ID NO：5所示; （c）由编码由（a）或（b）的多核苷酸编码的酶的片段或衍生物的核苷酸序列组成或包含的多核苷酸，其中所述衍生物中的一个氨基酸残基与氨基酸序列相比被保守地取代 的（a）; （d）编码具有羧酸酯酶活性的酶的多核苷酸与编码如SEQ ID NO：5所示的酶的多核苷酸至少66％相同; （e）由（a）至（b）中任一项所定义的多核苷酸在严格条件下与其互补的核苷酸序列杂交的核苷酸序列组成或包含的多核苷酸; 和（f）由（e）的多核苷酸的核苷酸序列退化的核苷酸序列组成或包含的多核苷酸; 或（a）至（f）的多核苷酸的互补链。

    A group of esterases for the enantioselective production of fine and speciality chemicals
    5.
    发明公开
    A group of esterases for the enantioselective production of fine and speciality chemicals 有权
    酯酶集团不对称生产精细和专用化学品

    公开(公告)号：EP2385109A3

    公开(公告)日：2012-02-15

    申请号：EP11002834.7

    申请日：2007-05-03

    申请人： B.R.A.I.N. Biotechnology Research And Information Network AG

    发明人： Liebeton, Klaus ,  Eck, Jürgen ,  Bornscheuer, Uwe ,  Böttcher, Dominique ,  Langer, Peter ,  Bellur, Esen

    IPC分类号： C12N9/18 ,  C12N15/55 ,  C07K16/40 ,  C12N5/10

    CPC分类号： C12N9/18

    摘要： The present invention relates to a polynucleotide consisting of more than 30 nucleotides encoding an enzyme having carboxylesterase [E.C. 3.1.1.1] activity, wherein the coding sequence is selected from the group consisting of (a) a polynucleotide encoding an amino acid sequence as depicted in any one of SEQ ID NOs: 10, 4; 8, and 12; (b) a polynucleotide consisting of or comprising a nucleotide sequence, wherein the nucleic acid sequence is as shown in any one of SEQ ID NOs: 9, 3, 7 and 11; (c) a polynucleotide consisting of or comprising a nucleotide sequence encoding a fragment or derivative of an enzyme encoded by a polynucleotide of (a) or (b), wherein in said derivative one amino acid residue is conservatively substituted compared to the amino acid sequence of (a); (d) a polynucleotide encoding an enzyme having carboxylesterase activity which polynucleotide is at least 66% identical to a polynucleotide encoding an enzyme as shown in any one of SEQ ID NOs: 10, 4, 8 and 12; (e) a polynucleotide consisting of or comprising a nucleotide sequence the complementary strand of which hybridizes under stringent conditions to a polynucleotide as defined in any one of (a) to (b); and (f) a polynucleotide consisting of or comprising a nucleotide sequence being degenerate to the nucleotide sequence of the polynucleotide of (e); or the complementary strand of such a polynucleotide of (a) to (f).