公开(公告)号：EP0986753B2

公开(公告)日：2005-08-10

申请号：EP98913541.3

申请日：1998-04-07

申请人： BioImage A/S

发明人： Thastrup, Ole ,  Bjorn, Sara Petersen ,  Tullin, Soren ,  Kasper, Almholt ,  Scudder, Kurt ,  Terry, Bernard Robert ,  Arkhammar, Per Olof Gunnar

IPC分类号： G01N33/53

CPC分类号： G01N33/5041 ,  G01N33/5005 ,  G01N33/5008 ,  G01N33/5014 ,  G01N33/5035 ,  G01N33/533 ,  G01N33/582 ,  G01N2333/43595 ,  G01N2333/9121

公开(公告)号：EP1143011B1

公开(公告)日：2005-10-26

申请号：EP01112115.9

申请日：1996-01-31

申请人： BioImage A/S

发明人： Thastrup, Ole ,  Tullin, Soren ,  Poulsen, Lars Kongsbak ,  Bjorn, Sara Petersen

IPC分类号： C12Q1/02 ,  G01N33/533 ,  C07K14/435 ,  C12N15/65 ,  G01N33/50

CPC分类号： G01N33/5008 ,  G01N33/502 ,  G01N33/533 ,  G01N2333/43595

公开(公告)号：EP1143011A3

公开(公告)日：2004-03-31

申请号：EP01112115.9

申请日：1996-01-31

申请人： BioImage A/S

发明人： Thastrup, Ole ,  Tullin, Soren ,  Poulsen, Lars Kongsbak ,  Bjorn, Sara Petersen

IPC分类号： C12Q1/02 ,  G01N33/533 ,  C07K14/435 ,  C12N15/65 ,  G01N33/50

CPC分类号： G01N33/5008 ,  G01N33/502 ,  G01N33/533 ,  G01N2333/43595

摘要： The resent invention relates to a method of characterizing the biological activity of a sample, the method comprising: (a) expressing a DNA sequence in a cell, wherein the DNA sequence encodes a protein or polypeptide selected from the group consisting of (i) a green fluorescent protein having a protein kinase recognition site, or (ii) a green fluorescent protein wherein one or more amino acids have been substituted, inserted or deleted to provide a binding domain of a second messenger, or (iii) a hybrid polypeptide of green fluorescent protein (GFP) or a modified GFP and a binding domain of a second messenger or an enzyme recognition site; and (b) measuring the fluorescence of said GFP in said cell in the absence and presence of a test sample; and (c) comparing the GFP fluorescence measured in step (b); wherein a difference between the fluorescence measured in the absence and presence of said sample is indicative of the biological activity of said test sample.

公开(公告)号：EP1143011A2

公开(公告)日：2001-10-10

申请号：EP01112115.9

申请日：1996-01-31

申请人： BioImage A/S

发明人： Thastrup, Ole ,  Tullin, Soren ,  Poulsen, Lars Kongsbak ,  Bjorn, Sara Petersen

IPC分类号： C12Q1/02 ,  C12N15/65 ,  C07K14/435

CPC分类号： G01N33/5008 ,  G01N33/502 ,  G01N33/533 ,  G01N2333/43595

摘要： The resent invention relates to a method of characterizing the biological activity of a sample, the method comprising: (a) expressing a DNA sequence in a cell, wherein the DNA sequence encodes a protein or polypeptide selected from the group consisting of (i) a green fluorescent protein having a protein kinase recognition site, or (ii) a green fluorescent protein wherein one or more amino acids have been substituted, inserted or deleted to provide a binding domain of a second messenger, or (iii) a hybrid polypeptide of green fluorescent protein (GFP) or a modified GFP and a binding domain of a second messenger or an enzyme recognition site; and (b) measuring the fluorescence of said GFP in said cell in the absence and presence of a test sample; and (c) comparing the GFP fluorescence measured in step (b); wherein a difference between the fluorescence measured in the absence and presence of said sample is indicative of the biological activity of said test sample.

摘要翻译： 本发明涉及表征样品的生物活性的方法，所述方法包括：（a）在细胞中表达DNA序列，其中所述DNA序列编码选自以下的蛋白质或多肽：（i） 绿色荧光蛋白具有蛋白激酶识别位点，或（ii）绿色荧光蛋白，其中一个或多个氨基酸被取代，插入或缺失以提供第二信使的结合结构域，或（iii）绿色荧光蛋白的杂合多肽 荧光蛋白（GFP）或修饰的GFP和第二信使或酶识别位点的结合结构域; 和（b）在不存在和存在测试样品的情况下测量所述细胞中所述GFP的荧光; 和（c）比较步骤（b）中测量的GFP荧光; 其中在不存在和存在所述样品时测量的荧光之间的差异指示所述测试样品的生物活性。