公开(公告)号：EP1003894A2

公开(公告)日：2000-05-31

申请号：EP98936923.6

申请日：1998-07-20

申请人： CHIRON CORPORATION

发明人： CHEN, Shin-Tai ,  GASMI, Mehdi ,  YEE, Jiing Kuan ,  JOLLY, Douglas, J.

IPC分类号： C12N15/86 ,  C12N5/10 ,  C12N7/01 ,  A61K48/00

CPC分类号： C12N15/86 ,  C12N7/00 ,  C12N2740/16021 ,  C12N2740/16043 ,  C12N2740/16052 ,  C12N2830/003 ,  C12N2830/42 ,  C12N2830/48 ,  C12N2830/60 ,  C12N2840/20 ,  C12N2840/203

摘要： Lentiviral vectors are provided comprising a 5' lentiviral LTR, a tRNA binding site, a packaging signal, a promoter operably linked to one or more genes of interest, an origin of second strand DNA synthesis and a 3' lentiviral LTR, wherein the lentiviral vector contains a nuclear transport element that is not RRE. Also provided are expression cassettes and packaging cell lines for producing lentiviral vector particles.