公开(公告)号：EP0343394A1

公开(公告)日：1989-11-29

申请号：EP89107582.2

申请日：1989-04-26

申请人： ENDOTRONICS INC.

发明人： Wilson, John Robert ,  Page, Darrell P. ,  Gaines, William A., Jr. ,  Harm, William H.

IPC分类号： C12M3/00 ,  C12M1/04

CPC分类号： C12M29/16 ,  C12M29/12 ,  Y10S435/818

摘要： A method of culturing cells by providing a highly gassed media on a continous basis to cells grown in a hollow fiber cartridge includes entraining the media with gas above the gas solubility level of the media. The media is then permitted to rest in a substantially quiescent state so that non-solubilized gas leaves the media. The media is then pressurized prior to delivery to the hollow fibers sufficiently so that the pressure drop and/or flow disturbance in the cell culturing device does not decrease the gas solubility level of the media below the media's gas saturation point.

摘要翻译： 通过连续的基础上，以在中空纤维筒中生长的细胞提供高度充气的培养基中培养细胞的方法，包括与所述媒体的气体溶解度水平以上气体夹带介质。 然后，媒体被允许休息静止状态基本上如此做了不溶解的气体离开媒体。 媒体然后输送到中空纤维之前加压充分所以做了压力降和/或流动扰动在细胞培养装置不降低媒体的气体饱和点以下的介质的气体溶解度水平。