公开(公告)号：EP1657312A1

公开(公告)日：2006-05-17

申请号：EP05023684.3

申请日：2005-10-28

申请人： EPPENDORF AG

发明人： Remacle, José ,  de Longueville, Françoise ,  Zammatteo, Nathalie ,  Toussaint, Olivier ,  van Huffel, Christophe

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6837 ,  C12Q1/6809 ,  C12Q2541/10 ,  C12Q2565/501

摘要： The invention provides a tool and a method for the easy interpretation of the changes occurring in a cell, being a three dimensional complex and control system when transfected by RNAi. The method is based on the analysis of a limited number of data obtained by quantifying the intensity of the signals present on spots distributed in a two dimensional surface. The invention allows to observe the effects of the presence of a particular RNAI on the cells main vital cellular functions together with the possible side or deleterious effects resulting from the transfection process or to the presence of the RNAi in a cell. The invention also provides a method for the determination of change in the three dimensional status of a cell, wherein an array containing nucleic acids or proteins belonging to or being representative for at least 5 vital cellular functions together with 3 deleterious functions

摘要翻译： 本发明提供了一种工具，用于变化在细胞中发生的，是一个三维复杂及控制系统当通过RNAi转染的容易解释的方法。 该方法是基于通过定量本信号的强度分布上在二维表面斑点获得的数据的有限数目的分析。 本发明允许与可能的副作用或有害的从转染过程或所述RNAi在细胞中存在而产生的观察特定RNAI的细胞上的存在的影响，主要重要的细胞功能在一起。 因此本发明提供了一种用于改变在细胞的三维状态的确定，来worin阵列含有核酸或为具有3个有害功能属于或代表被至少五种重要的细胞功能的蛋白一起