公开(公告)号：EP0420358A1

公开(公告)日：1991-04-03

申请号：EP90202565.9

申请日：1990-09-27

申请人： GIST-BROCADES N.V.

发明人： van Gorcom, Robert Franciscus Maria ,  van Hartingsveldt, Willem ,  van Paridon, Peter Andreas ,  Veenstra, Annemarie Eveline ,  Luiten, Rudolf Gijsbertus Marie ,  Selten, Gerardus Cornelis Maria

IPC分类号： C12N15/55 ,  C12N9/16 ,  A23K1/165 ,  C12N1/15 ,  C12N1/19

CPC分类号： C12N9/16 ,  A23K20/189

摘要： A nucleotide sequence encoding phytase has been isolated and cloned. The coding sequence has been inserted into an expression construct which in turn has been inserted into a vector capable of transforming a microbial expression host. The transformed microbial hosts may be used to economically produce phytase on an industrial scale. The phytase produced via the present invention may be used in a variety of processes requiring the conversion of phytate to inositol and inorganic phosphate.

摘要翻译： 已经分离和克隆编码植酸酶的核苷酸序列。 编码序列已被插入到表达构建体中，该构建体又被插入到能够转化微生物表达宿主的载体中。 转化的微生物宿主可用于在工业规模上经济地生产植酸酶。 通过本发明产生的植酸酶可以用于需要将肌醇六磷酸转化成肌醇和无机磷酸盐的各种方法。