公开(公告)号：EP1546387B1

公开(公告)日：2016-01-06

申请号：EP03752373.5

申请日：2003-09-12

申请人： Gen9, Inc.

发明人： OLEINIKOV, Andrew, V.

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12N15/1068 ,  B01J19/0046 ,  B01J2219/00378 ,  B01J2219/00385 ,  B01J2219/00432 ,  B01J2219/00454 ,  B01J2219/005 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/00626 ,  B01J2219/00639 ,  B01J2219/00641 ,  B01J2219/00659 ,  B01J2219/00675 ,  B01J2219/00677 ,  B01J2219/00689 ,  B01J2219/00713 ,  B01J2219/00722 ,  B82Y30/00 ,  C12Q1/6837 ,  C40B40/06 ,  C40B50/14 ,  C40B80/00

摘要： The invention relates to a process for assembling a polynucleotide from a plurality of oligonucleotides comprising: (a) synthesizing or spotting a plurality of different oligonucleotides on a microarray device or bead device having a solid or porous surface, wherein the plurality of oligonucleotides are attached to the solid or porous surface, and wherein each oligonucleotide comprises a sequence region of from about 10 to about 50 bases that is the same or substantially the same as a sequence region of another oligonucleotide; (b) forming an oligonucleotide complementary to a first oligonucleotide by extending a first primer, wherein said primer is complementary to a portion of said first oligonucleotide; (c) is associating said complementary oligonucleotide from said first oligonucleotide and annealing said complementary oligonucleotide to another oligonucleotide comprising a sequence region that is the same or substantially the same as a sequence region of said first oligonucleotide; (d) repeating the primer extension cycles of step (c) to produce a full-length complementary polynucleotide; and (e) amplifying the assembled full length complementary polynucleotide to produce a full length polynucleotide in desired quantities.

公开(公告)号：EP3059321B1

公开(公告)日：2020-08-05

申请号：EP15189572.9

申请日：2003-09-12

申请人： Gen9, Inc.

发明人： OLEINIKOV, Andrew, V.

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

公开(公告)号：EP3059321A1

公开(公告)日：2016-08-24

申请号：EP15189572.9

申请日：2003-09-12

申请人： Gen9, Inc.

发明人： OLEINIKOV, Andrew, V.

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

摘要： There is disclosed a process for in vitro synthesis and assembly of long, gene-length polynucleotides based upon assembly of multiple shorter oligonucleotides synthesized in situ on a microarray platform. Specifically, there is disclosed a process for in situ synthesis of oligonucleotide fragments on a solid phase microarray platform and subsequent, "on device" assembly of larger polynucleotides composed of a plurality of shorter oligonucleotide fragments.

摘要翻译： 公开了一种基于在微阵列平台上原位合成的多个较短的寡核苷酸的组装体外合成和组装长的基因长度多核苷酸的方法。 具体地，公开了在固相微阵列平台上原位合成寡核苷酸片段的方法，随后在由多个较短的寡核苷酸片段组成的较大多核苷酸的“器件”装配上。