公开(公告)号：EP1350840A1

公开(公告)日：2003-10-08

申请号：EP02076334.8

申请日：2002-04-05

申请人： ID-Lelystad, Instituut voor Dierhouderij en Diergezondheid B.V.

发明人： van Rijn, Petrus Antonius ,  Meulenberg, Johanna Jacoba Maria

IPC分类号： C12N7/01 ,  C12N7/04 ,  C07K14/08

CPC分类号： C07K14/005 ,  A61K2039/522 ,  C12N7/00 ,  C12N2770/10021 ,  C12N2770/10022 ,  C12N2770/10061 ,  C12N2770/10064

摘要： The invention relates to the field of virology , more in particular to the field of vaccine production, more specifically to the in vitro propagation of virus, more specifically to the adaptation of virus to a cell line.
The invention provides a method to determine the capability of an arterivirus to replicate in a green monkey cell line, comprising determining the amino acids at positions, which correspond to the amino acid positions 75-107 of GP2a of PRRSV isolate I-1102, more specifically amino acid position 88 and/or amino acid position 95 of said GP2a. The invention further discloses a method to produce arterivirus in green monkey cell line wherein the virulence of said arterivirus is maintained, with increased virus yield.
The invention further provides a method to determine the attenuation of an arterivirus, comprising determining the amino acids at positions, which correspond to the amino acid positions 121-148 of GP5 of PRRSV isolate I-1102, more specifically amino acid 136 of said GP5 and/or determining the amino acids at positions, which correspond to the amino acid positions 651-675 and/or amino acid positions 2331-2355 of ORF1ab of PRRSV isolate I-1102, more specifically amino acid 663 and/or 2343 of said ORF1ab. The invention also provides a method to attenuate the virulence of said arterivirus by changing said amino acids.

摘要翻译： 本发明涉及病毒学领域，更具体地涉及疫苗生产领域，更具体地涉及病毒的体外繁殖，更具体地涉及将病毒适应于细胞系。 本发明提供一种确定动物病毒在绿猴细胞系中复制的能力的方法，其包括确定与PRRSV分离株I的GP2a的氨基酸位置75-107相对应的位置处的氨基酸， 1102，更具体地说是GP2a的氨基酸位置88和/或氨基酸位置95。 本发明还公开了一种在绿猴细胞系中产生动脉病毒的方法，其中维持所述动脉病毒的毒力，病毒产量增加。 本发明进一步提供了确定动脉病毒衰减的方法，其包括确定对应于PRRSV分离物I-1102的GP5的氨基酸位置121-148的位置的氨基酸，更具体地说，氨基酸136 的所述GP5和/或确定对应于PRRSV分离物I-1102的ORF1ab的氨基酸位置651-675和/或氨基酸位置2331-2355的位置的氨基酸，更具体地，氨基酸663和/或2343 的ORF1ab。 本发明还提供了通过改变所述氨基酸来减弱所述动脉病毒的毒力的方法。