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1.发明公开Method of preparing primers for polymerase chain reaction and DNA amplification using said primers 失效一种制备引物的聚合酶链反应和扩增的DNA通过使用这些引物的过程。
公开(公告)号：EP0438292A2
公开(公告)日：1991-07-24
申请号：EP91300326.5
申请日：1991-01-17
CPC分类号： C12N15/10
摘要： Primers for a polymerase chain reaction are prepared by treating a double-standard DNA fragment with an exonuclease. DNA is amplified in vitro by the polymerase chain reaction using such primers. Chromosomal genes are amplified by carrying out the inverted polymerase chain reaction using as a primer, a DNA fragment obtained by treating a double-stranded DNA fragment with an exonuclease capable of digesting one chain of the double-stranded DNA in a manner such that mononucleotides are released therefrom in the direction from the 5′ terminus to the 3′ terminus and, as a template, a circular DNA obtained by cleaving the chromosomal gene with a restriction enzyme and subjecting the resultant fragment to self-ligation.
摘要翻译： 引物聚合酶链式反应通过在核酸外切酶处理的双标准DNA片段来制备。 DNA在体外通过使用引物检测聚合酶链式反应扩增。 染色体基因是通过使用作为引物，通过在能够以这样的方式搜索没有单核苷酸是消化双链DNA的一条链的核酸外切酶处理双链DNA片段而获得的DNA片段进行反转聚合酶链反应扩增 从5分钟末端到3个分钟末端释放那里从在方向和作为模板，通过切割用限制性酶的染色体基因并将所得片段自身连接得到的环状DNA。
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2.发明公开Method of preparing primers for polymerase chain reaction and DNA amplification using said primers 失效制备聚合酶链反应的引物的方法和使用上述引物的DNA扩增
公开(公告)号：EP0438292A3
公开(公告)日：1991-09-18
申请号：EP91300326.5
申请日：1991-01-17
CPC分类号： C12N15/10
摘要： Primers for a polymerase chain reaction are prepared by treating a double-standard DNA fragment with an exonuclease. DNA is amplified in vitro by the polymerase chain reaction using such primers. Chromosomal genes are amplified by carrying out the inverted polymerase chain reaction using as a primer, a DNA fragment obtained by treating a double-stranded DNA fragment with an exonuclease capable of digesting one chain of the double-stranded DNA in a manner such that mononucleotides are released therefrom in the direction from the 5′ terminus to the 3′ terminus and, as a template, a circular DNA obtained by cleaving the chromosomal gene with a restriction enzyme and subjecting the resultant fragment to self-ligation.
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3.发明授权Method of preparing primers for polymerase chain reaction and DNA amplification using said primers 失效通过使用这些引物用于聚合酶链反应和DNA扩增引物的制备方法
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